Food Industry FAMEs on Rt-2560 by AOCS Method Ce-1j-07 Using H2 (Optimized Method)

GC_FF1348

	Peaks	t_R (min)	Conc. (µg/mL)	Structural Nomenclature
1.	Methyl caproate	4.740	400	C6:0
2.	Methyl octanoate	4.953	400	C8:0
3.	Methyl decanoate	5.317	400	C10:0
4.	Methyl undecanoate	5.587	200	C11:0
5.	Methyl dodecanoate	5.939	400	C12:0
6.	Methyl tridecanoate	6.398	200	C13:0
7.	Methyl myristate	6.998	400	C14:0
8.	Methyl myristoleate	7.736	200	C14:1 (c9)
9.	Methyl pentadecanoate	7.781	200	C15:0
10.	Methyl pentadecenoate	8.734	200	C15:1 (C10)
11.	Methyl palmitate	8.806	600	C16:0
12.	Methyl palmitoleate	9.831	200	C16:1 (c9)
13.	Methyl heptadecanoate	10.136	200	C17:0
14.	Methyl heptadecenoate	11.448	200	C17:1 (c10)
15.	Methyl stearate	11.882	400	C18:0
16.	Methyl octadecenoate	12.877	200	C18:1 (t9)
17.	Methyl oleate	13.345	400	C18:1 (c9)
18.	Methyl linolelaidate	14.685	200	C18:2 (t9,t12)

	Peaks	t_R (min)	Conc. (µg/mL)	Structural Nomenclature
19.	Methyl linoleate	15.928	200	C18:2 (c9,c12)
20.	Methyl arachidate	17.136	400	C20:0
21.	Methyl linolenate	18.252	200	C18:3 (c6,c9,c12)
22.	Methyl eicosenoate	19.422	200	C20:1 (c11)
23.	Methyl linolenate	19.740	200	C18:3 (c9,c12,c15)
24.	Methyl heneicosanoate	20.592	200	C21:0
25.	Methyl eicosadienoate	21.621	200	C20:2 (c11,c14)
26.	Methyl behenate	22.380	400	C22:0
27.	Methyl eicosatrienoate	22.884	200	C20:3 (c8,c11,c14)
28.	Methyl erucate	23.510	200	C22:1 (c13)
29.	Methyl eicosatrienoate	23.656	200	C20:3 (c11,c14,c17)
30.	Methyl arachidonate	23.901	200	C20:4 (c5,c8,c11,c14)
31.	Methyl tricosanoate	24.240	200	C23:0
32.	Methyl docosadienoate	25.408	200	C22:2 (c13,c16)
33.	Methyl eicosapentaenoate	26.293	200	C20:5 (c5,c8,c11,c14,c17)
34.	Methyl lignocerate	26.293	400	C24:0
35.	Methyl nervonate	27.653	200	C24:1 (c15)
36.	Methyl docosahexaenoate	33.053	200	C22:6 (c4,c7,c10,c13,c16,c19)

Injection
Column	Rt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/Sample	Food industry FAME mix (cat.# 35077)

Diluent:	Hexane
Conc.:	10,000 µg/mL total concentration
Inj. Vol.:	1 µL split (split ratio 20:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	180 °C (hold 19.8 min) to 215 °C at 32.3 °C/min (hold 11 min)
Carrier Gas	H₂, constant flow
Flow Rate:	2.5 mL/min

Detector	FID @ 250 °C
Constant Column + Constant Make-up:	52 mL/min
Hydrogen flow:	40 mL/min
Air flow:	400 mL/min
Data Rate:	50 Hz
Instrument	Agilent 7890A GC
Notes	Method optimized from He using the EZGC method translator (www.restek.com/FAMEH₂). C4:0 Methyl butyrate (623-42-7) elutes in the solvent front. Resolution of critical pair cis-11-C20:1 (#22) and cis-9,12,15-C18:3 (#23) is 2.03.

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