Food Industry FAMEs on Rt-2560 by AOCS Method Ce-1h-05

GC_FF1349

	Peaks	t_R (min)	Conc. (µg/mL)	Structural Nomenclature
1.	Methyl caproate	5.830	400	C6:0
2.	Methyl octanoate	6.092	400	C8:0
3.	Methyl decanoate	6.539	400	C10:0
4.	Methyl undecanoate	6.870	200	C11:0
5.	Methyl dodecanoate	7.302	400	C12:0
6.	Methyl tridecanoate	7.866	200	C13:0
7.	Methyl myristate	8.603	400	C14:0
8.	Methyl myristoleate	9.509	200	C14:1 (c9)
9.	Methyl pentadecanoate	9.563	200	C15:0
10.	Methyl pentadecenoate	10.733	200	C15:1 (C10)
11.	Methyl palmitate	10.822	600	C16:0
12.	Methyl palmitoleate	12.080	200	C16:1 (c9)
13.	Methyl heptadecanoate	12.453	200	C17:0
14.	Methyl heptadecenoate	14.065	200	C17:1 (c10)
15.	Methyl stearate	14.597	400	C18:0
16.	Methyl octadecenoate	15.817	200	C18:1 (t9)
17.	Methyl oleate	16.393	400	C18:1 (c9)
18.	Methyl linolelaidate	18.037	200	C18:2 (t9,t12)

	Peaks	t_R (min)	Conc. (µg/mL)	Structural Nomenclature
19.	Methyl linoleate	19.563	200	C18:2 (c9,c12)
20.	Methyl arachidate	21.048	400	C20:0
21.	Methyl linolenate	22.417	200	C18:3 (c6,c9,c12)
22.	Methyl eicosenoate	23.849	200	C20:1 (c11)
23.	Methyl linolenate	24.242	200	C18:3 (c9,c12,c15)
24.	Methyl heneicosanoate	25.794	200	C21:0
25.	Methyl eicosadienoate	29.073	200	C20:2 (c11,c14)
26.	Methyl behenate	32.095	400	C22:0
27.	Methyl eicosatrienoate	33.613	200	C20:3 (c8,c11,c14)
28.	Methyl erucate	36.531	200	C22:1 (c13)
29.	Methyl eicosatrienoate	36.727	200	C20:3 (c11,c14,c17)
30.	Methyl arachidonate	37.548	200	C20:4 (c5,c8,c11,c14)
31.	Methyl tricosanoate	40.227	200	C23:0
32.	Methyl docosadienoate	45.045	200	C22:2 (c13,c16)
33.	Methyl eicosapentaenoate	48.043	200	C20:5 (c5,c8,c11,c14,c17)
34.	Methyl lignocerate	51.086	400	C24:0
35.	Methyl nervonate	58.002	200	C24:1 (c15)
36.	Methyl docosahexaenoate	86.473	200	C22:6 (c4,c7,c10,c13,c16,c19)

Injection
Column	Rt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/Sample	Food industry FAME mix (cat.# 35077)

Diluent:	Hexane
Conc.:	10,000 µg/mL total concentration
Inj. Vol.:	1 µL split (split ratio 20:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	180 °C (hold 90 min)
Carrier Gas	H₂, constant flow
Flow Rate:	1.75 mL/min

Detector	FID @ 250 °C
Constant Column + Constant Make-up:	52 mL/min
Hydrogen flow:	40 mL/min
Air flow:	400 mL/min
Data Rate:	50 Hz
Instrument	Agilent 7890A GC
Notes	Hydrogen flow optimized to achieve effective linear velocity (www.restek.com/FAMEH₂). C4:0 Methyl butyrate (623-42-7) elutes in the solvent front.

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