Butylated Hydroxytoluene (BHT) and Butylated Hydroxyanisole (BHA) on Rtx-50

	Peaks	t_R (min)	Conc. (µg/mL)	Mass On-Column (ng)
1.	Butylated hydroxytoluene (BHT)	7.35	2000	20
2.	Butylated hydroxyanisole (BHA)	7.83	4000	40

Injection
Column	Rtx-50, 30 m, 0.53 mm ID, 0.50 µm (cat.# 10540)
Standard/Sample	Butylated hydroxyanisole (BHA)
	Butylated hydroxytoluene (BHT)
Diluent:	Methanol
Inj. Vol.:	1 µL split (split ratio 100:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	250 °C
Split Vent Flow Rate:	859 mL/min
Gas Saver Time:	2 min
Gas Saver Flow Rate:	20 mL/min
Oven
Oven Temp.:	50 °C to 240 °C at 15 °C/min (hold 3 min)
Carrier Gas	He, constant pressure (6 psi, 41.4 kPa)
Linear Velocity:	58 cm/sec @ 50 °C

Detector	FID @ 250 °C
Make-up Gas Flow Rate:	35 mL/min
Make-up Gas Type:	H₂
Hydrogen flow:	40 mL/min
Air flow:	400 mL/min
Data Rate:	20 Hz
Instrument	Agilent 7890B GC
Sample Preparation	Weighed 0.002 g BHT and 0.004 g BHA and diluted to 1 mL in methanol, producing a mixed standard that contained 2000 μg/mL BHT and 4000 μg/mL BHA.

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