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Butylated Hydroxytoluene (BHT) and Butylated Hydroxyanisole (BHA) on Rtx-50

PeakstR (min)Conc.
Mass On-Column (ng)
1.Butylated hydroxytoluene (BHT)7.35200020
2.Butylated hydroxyanisole (BHA)7.83400040
ColumnRtx-50, 30 m, 0.53 mm ID, 0.50 µm (cat.# 10540)
Standard/SampleButylated hydroxyanisole (BHA)
Butylated hydroxytoluene (BHT)
Inj. Vol.:1 µL split (split ratio 100:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:250 °C
Split Vent Flow Rate:859 mL/min
Gas Saver Time:2 min
Gas Saver Flow Rate:20 mL/min
Oven Temp.:50 °C to 240 °C at 15 °C/min (hold 3 min)
Carrier GasHe, constant pressure (6 psi, 41.4 kPa)
Linear Velocity:58 cm/sec @ 50 °C
DetectorFID @ 250 °C
Make-up Gas Flow Rate:35 mL/min
Make-up Gas Type:H2
Hydrogen flow:40 mL/min
Air flow:400 mL/min
Data Rate:20 Hz
InstrumentAgilent 7890B GC
Sample PreparationWeighed 0.002 g BHT and 0.004 g BHA and diluted to 1 mL in methanol, producing a mixed standard that contained 2000 μg/mL BHT and 4000 μg/mL BHA.