3-Monochloropropane-1,2-Diol (3-MCPD) Esters in Olive Oil on Rxi-17Sil MS

	Peaks	t_R (min)	Conc. (µg/g)
1.	3-MCPD-d5 PBA derivative	5.937	1.81
2.	3-MCPD PBA derivative	5.977	2.94

Injection
Column	Rxi-17Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 14123)
Standard/Sample	Extracted olive oil
Diluent:	Isooctane
Inj. Vol.:	1 µL split (split ratio 10:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	280 °C
Oven
Oven Temp.:	120 °C (hold 0.5 min) to 180 °C at 12 °C/min to 330 °C at 25 °C/min (hold 5 min)
Carrier Gas	He, constant flow
Flow Rate:	1.4 mL/min

Detector	MS
Mode:	SIM
SIM Program:	147, 150, 196, 201 m/z, 50 ms dwell
Transfer Line Temp.:	320 °C
Analyzer Type:	Quadrupole
Source Temp.:	230 °C
Quad Temp.:	150 °C
Instrument	Agilent 7890A GC & 5975C MSD
Sample Preparation	The olive oil sample was spiked with 3-MCPD-d5 ester and prepared according to AOCS method Cd 29c-13.

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