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Sugar TMS oximes on Rtx-1701

ColumnRtx-1701, 30 m, 0.25 mm ID, 0.25 µm (cat.# 12023)
Standard/SampleSugar TMS oximes
Diluent:Ethyl acetate
Conc.: 1-5 mg of starting material
Injection
Inj. Vol.:1 µL split (split ratio 100:1)
Liner:Topaz 4.0 mm ID Precision Inlet Liner w/ Wool (cat.# 23305)
Inj. Temp.:250 °C
Oven
Oven Temp.:40 °C (hold 1 min) to 280 °C at 19 °C/min
Carrier GasHe, constant flow
Flow Rate:1.4 mL/min
Linear Velocity:45.023 cm/sec @ 150 °C
Dead Time:1.11 min @ 150 °C
DetectorMS
Mode:Scan
Scan Program:
GroupStart Time
(min)
Scan Range
(amu)
Scan Rate
(scans/sec)
11.550-7507.5
Transfer Line Temp.:280 °C
Analyzer Type:Quadrupole
Source Type:Extractor
Source Temp.:280 °C
Quad Temp.:150 °C
Electron Energy:977 eV
Solvent Delay Time:1.5 min
Tune Type:PFTBA
Ionization Mode:EI
InstrumentAgilent 7890B GC & 5977A MSD
NotesApproximately 1 mg of sugar was dissolved in 100 μL of 40 mg/mL ethylhydroxylaminehydrochloride (EtOx) and heated at 70°C for 30 min. Samples were cooled to room temperature for approximately 5 min. 120 μL of N-Methyl-bis(trifluoroacetamide) (MBTFA) was added to each sample and then heated at 70°C for 30 min. Samples were diluted 1:100 in ethyl acetate.