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Peaks | |
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1. | 5-α-Androstane |
2. | Androsterone |
3. | Cholestane |
4. | Cholesterol |
5. | Stigmasterol |
Column | 3% OV-17, 100/120 mesh on DiatoSorb WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID |
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Standard/Sample | 5- |
Androsterone, 5α-cholestane, cholesterol, and stigmasterol purchased from Sigma-Aldrich | |
Conc.: | 2.0 µg/mL |
Injection | |
Inj. Vol.: | 0.5 µL on-column |
Inj. Temp.: | 230 °C |
Oven | |
Oven Temp.: | 250 °C (hold 17 min) |
Carrier Gas | He, constant flow |
Flow Rate: | 40 mL/min |
Detector | FID @ 275 °C |
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Instrument | Agilent 7890B GC |
Notes |
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