Steroids on DiatoSorb WHP Packed Column

Injection
Column	3% OV-17, 100/120 mesh on DiatoSorb WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID
Sample	5-α-Androstane (cat.# 31065)
	Androsterone, 5α-cholestane, cholesterol, and stigmasterol purchased from Sigma-Aldrich
Conc.:	2.0 µg/mL
Inj. Vol.:	0.5 µL on-column
Inj. Temp.:	230 °C
Oven
Oven Temp.:	250 °C (hold 17 min)
Carrier Gas	He, constant flow
Flow Rate:	40 mL/min

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