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Steroids on DiatoSorb WHP Packed Column

Column3% OV-17, 100/120 mesh on DiatoSorb WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID
Sample5-α-Androstane (cat.# 31065)
Androsterone, 5α-cholestane, cholesterol, and stigmasterol purchased from Sigma-Aldrich
Conc.:2.0 µg/mL
Inj. Vol.:0.5 µL on-column
Inj. Temp.:230 °C
Oven Temp.:250 °C (hold 17 min)
Carrier GasHe, constant flow
Flow Rate:40 mL/min
DetectorFID @ 275 °C
InstrumentAgilent 7890B GC