Aromatic Amino Acids on Ultra Aqueous C18

LC_0146

	Peaks	Conc. (mg/mL)
1.	Tyrosine	0.2
2.	Phenylalanine	1.6
3.	Tryptophan	0.4

Column

Ultra Aqueous C18 (cat.# 9178565)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Diluent:

mobile phase A

Inj. Vol.:

20 µL

Mobile Phase

A:

50 mM potassium phosphate, pH 2.5

B:

acetonitrile

Time (min)	%B
0–5	5–20
5–6	20–5
6–13	Hold at 5

Flow:

1.0 mL/min

Detector	UV/Vis @ 254 nm

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