Capsaicinoids: Heat Level Assay for Habeñero Nuggets on Ultra C18

LC_0156

	Peaks
1.	Unknown
2.	Capsaicin
3.	Dihydrocapsaicin

Column

Ultra C18 (cat.# 9174565)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

Ambient

Standard/Sample

Habañero nugget extract

Diluent:

Ethanol

Conc.:

0.8 g sample in 200 mL

Inj. Vol.:

10 µL

Mobile Phase

A:

1% Acetic acid in water

B:

Acetonitrile

Time (min)	%B
0.00	50
2	50
9	80
12	80
13	50

Flow:

1.0 mL/min

Detector	UV/Vis @ 280 nm

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