Aromatic Amino Acids on Ultra Aqueous C18 (Fast Analysis)

LC_0307

	Peaks	t_R (min)	Conc. (mg/mL)
1.	Tyrosine	1.286	1.25
2.	Phenylalanine	2.197	0.55
3.	Tryptophan	2.475	0.035

Column

Ultra Aqueous C18 (cat.# 9178535)

Dimensions:

30 mm x 4.6 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

50 °C

Standard/Sample

Diluent:

50mM potassium phosphate, pH 2.5

Inj. Vol.:

10 µL

Mobile Phase

A:

50mM potassium phosphate, pH 2.5

B:

acetonitrile

Time (min)	%A	%B
0.00	100	0
1.0	60	40
1.2	100	0
5.0	100	0

Flow:

1.0 mL/min

Detector	UV/Vis @ 254 nm

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