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Peaks | tR (min) | Prec. Ion | Prod. Ion | Collision E (V) | Tube Lens | |
---|---|---|---|---|---|---|
1. | d5-AMOZ | 3.7 | -- | -- | -- | -- |
2. | AMOZ | 3.8 | 335 | 291 | 10 | 100 |
3. | AHD | 5.46 | 249 | 134 | 12 | 110 |
4. | d4-AOZ | 5.49 | -- | -- | -- | -- |
5. | AOZ | 5.51 | 236 | 134 | 12 | 120 |
6. | SC | 5.6 | 209 | 166 | 12 | 80 |
Column | Ultra C18 (cat.# 9174312) | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||
Particle Size: | 3 µm | ||||||||||||||
Pore Size: | 100 Å | ||||||||||||||
Temp.: | 30 °C | ||||||||||||||
Standard/Sample | |||||||||||||||
Conc.: | 0.3 ppb each analyte | ||||||||||||||
Mobile Phase | |||||||||||||||
A: | 0.05% formic acid in methanol | ||||||||||||||
B: | 0.05% formic acid – 5 mM NH4 formate in water | ||||||||||||||
| |||||||||||||||
Flow: | 0.2 mL/min |
Detector | MS/MS triple quadrupole (Thermo Scientific Discovery) |
---|---|
Ion Source: | Electrospray |
Ion Mode: | ESI+ |
Notes | Analyzer Parameters: Only segment: 15 min Data type: centroid Scan mode: SRM product Scan width (m/z): 0.7 Scan time (s): 0.25 Peak width: Q1: within 0.7; Q2: 0.7 Collision gas pressure (mTorr): 1.5 (argon) Divert valve: active, with 3 positions Positions-1° 2 min, 2° 8 min, 3° 5 min |
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