Nitrofuran Metabolites by LC-MS/MS on Ultra C18 Column

	Peaks	t_R (min)	Prec. Ion	Prod. Ion	Collision E (V)	Tube Lens
1.	d5-AMOZ	3.7	--	--	--	--
2.	AMOZ	3.8	335	291	10	100
3.	AHD	5.46	249	134	12	110
4.	d4-AOZ	5.49	--	--	--	--
5.	AOZ	5.51	236	134	12	120
6.	SC	5.6	209	166	12	80

AMOZ = 3-amino-5-morpholinomethyl-2-oxazolidinone; AHD = 1-aminohydantoin hydrochloride; AOZ = 3-amino-2-oxazolidinone; SC = semicarbazide

Column

Ultra C18 (cat.# 9174312)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Conc.:

0.3 ppb each analyte

Mobile Phase

0.05% formic acid in methanol

0.05% formic acid – 5 mM NH₄ formate in water

Flow:

0.2 mL/min

Detector	MS/MS triple quadrupole (Thermo Scientific Discovery)
Ion Source:	Electrospray
Ion Mode:	ESI+
Notes	Analyzer Parameters: Only segment: 15 min Data type: centroid Scan mode: SRM product Scan width (m/z): 0.7 Scan time (s): 0.25 Peak width: Q1: within 0.7; Q2: 0.7 Collision gas pressure (mTorr): 1.5 (argon) Divert valve: active, with 3 positions Positions-1° 2 min, 2° 8 min, 3° 5 min

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