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|Column||Raptor C18 (cat.# 9304A65)|
|Dimensions:||150 mm x 4.6 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Standard/Sample||Laboratory air sample|
|Diluent:||2 mL acetonitrile extract|
|Inj. Vol.:||5 µL|
|Detector||UV/Vis @ 365, 4.8 nm|
|Instrument||Waters ACQUITY UPLC H-Class|
|Sample Preparation||The radiello 165 passive air sampler (RAD165) utilizes a stainless steel net cartridge filled with 2,4-dinitrophenylhydrazine (DNPH) coated Florisil adsorbent. Airborne carbonyls react with the DNPH to form stable hydrazones. The derivatives were subsequently extracted for 30 minutes with 2 mL of acetonitrile. The extract was then analyzed by LC-UV.|
|Notes||*Mobile phase B was prepared by combining 650 mL methanol and 50 mL acetonitrile.|