Analysis of Taxanes on Raptor FluoroPhenyl by LC-MS/MS

LC_BA0351

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Baccatin III	0.97	587.0	405.1	105.0
2.	Docetaxel	1.25	808.1	527.3	226.1
3.	Paclitaxel	1.33	854.1	569.3	286.2

Column

Raptor FluoroPhenyl (cat.# 931955E)

Dimensions:

50 mm x 3 mm ID

Particle Size:

5 µm

Temp.:

35 °C

Standard/Sample

Diluent:

Water

Conc.:

100 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	75	25
2.00	0.8	5	95
2.01	0.8	75	25
3.50	0.8	75	25

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC

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