Drugs of Abuse on Ultra Biphenyl

	Peaks	t_R (min)	Positive MRM1	Positive MRM2	Positive MRM3	Negative MRM1	Negative MRM2	Negative MRM3
1.	Ecgonine	0.58	186.1/168.2	186.1/150.9	186.1/82.2	--	--	--
2.	Ecgonine methyl ester	0.62	200.2/82.2	200.2/68.2	200.2/65.1	--	--	--
3.	Morphine	1.35	286.1/201.3	286.1/229.9	286.1/181.1	--	--	--
4.	Amphetamine	1.63	136.0/91.0	136.0/119.1	136.0/65.0	--	--	--
5.	Codeine	1.76	300.2/165.2	300.2/152.2	300.2/115.2	--	--	--
6.	Methamphetamine	1.80	150.0/91.2	150.0/119.0	150.0/65.0	--	--	--
7.	6-MAM	1.88	328.1/165.1	328.1/211.1	328.1/152.2	--	--	--
8.	MDMA	1.94	194.1/163.1	194.1/105.1	194.1/133.0	--	--	--
9.	Benzoylecgonine	2.14	290.1/168.1	290.1/105.1	290.1/77.1	--	--	--
10.	Cocaine	2.59	304.2/182.1	304.2/82.1	304.2/105.0	--	--	--
11.	Cocaethylene	2.84	318.2/196.2	318.2/82.2	318.2/77.1	--	--	--
12.	PCP	3.02	244.2/86.1	244.2/159.2	244.2/91.1	--	--	--
13.	THC-COOH	4.27	--	--	--	343.0/299.2	343.0/190.8	343.0/245.4
14.	THC	4.63	315.3/193.3	315.3/93.0	315.3/69.1	--	--	--

The instrument was operated in multi-period mode for this experiment. Period 1 was ESI+, lasting from 0.0 min to 3.5 min, Period 2 was ESI-, lasting from 3.5 min to 4.5 min, Period 3 was ESI+, lasting from 4.5 min to 6.5 min

Column

Ultra Biphenyl (cat.# 9109552)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Diluent:

0.1 Formic acid in water:0.1% formic acid in acetonitrile (98:2)

Conc.:

50 ng/mL

Inj. Vol.:

10 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	98	2
2.7	0.6	60	40
4.5	0.6	0	100
5.0	0.6	0	100
5.1	0.6	98	2

Detector	Applied Biosystems/MDS Sciex LC-MS/MS
Model #:	API 4000
Ion Source:	TurboIonSpray^®
Ion Spray Voltage:	+/- 4.5 kV
Curtain Gas:	40 psi (275.8 kPa)
Gas 1:	60 psi (413.7 kPa)
Gas 2:	60 psi (413.7 kPa)
Interface Temp.:	600 °C
Mode:	MRM
Instrument	Applied Biosystems/MDS Sciex LC-MS/MS System

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