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Peaks | tR (min) | Conc. (µg/mL) | |
---|---|---|---|
1. | Cortisol | 0.74 | 25 |
2. | Corticosterone | 1.31 | 25 |
3. | Estradiol | 1.49 | 100 |
4. | Boldenone | 1.66 | 25 |
5. | Trenbolone | 1.84 | 25 |
Peaks | tR (min) | Conc. (µg/mL) | |
---|---|---|---|
6. | Testosterone | 2.05 | 25 |
7. | Methyltestosterone | 2.29 | 25 |
8. | 17-OH-progesterone | 2.46 | 25 |
9. | Androstenedione | 3.02 | 25 |
10. | Progesterone | 4.48 | 25 |
11. | 25-Hydroxyvitamin D3 | 4.69 | 25 |
Column | Raptor Biphenyl (cat.# 9309A12) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | Water:acetonitrile (60:40) | ||||||||||||||||||||||||
Conc.: | 25-100 µg/mL | ||||||||||||||||||||||||
Inj. Vol.: | 1 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | Water | ||||||||||||||||||||||||
B: | Acetonitrile | ||||||||||||||||||||||||
|
Detector | Waters Acquity® PDA @ 254 nm |
---|---|
Instrument | Waters ACQUITY UPLC H-Class |
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