Steroids on Raptor Biphenyl

LC_CF0559

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Cortisol	0.74	25
2.	Corticosterone	1.31	25
3.	Estradiol	1.49	100
4.	Boldenone	1.66	25
5.	Trenbolone	1.84	25

	Peaks	t_R (min)	Conc. (µg/mL)
6.	Testosterone	2.05	25
7.	Methyltestosterone	2.29	25
8.	17-OH-progesterone	2.46	25
9.	Androstenedione	3.02	25
10.	Progesterone	4.48	25
11.	25-Hydroxyvitamin D3	4.69	25

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

30 °C

Sample

Diluent:

Water:acetonitrile (60:40)

Conc.:

25-100 µg/mL

Inj. Vol.:

1 µL

Mobile Phase

A:

Water

B:

Acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	60	40
2.50	0.5	55	45
5.00	0.5	0	100
5.01	0.5	60	40
7.00	0.5	60	40

Detector	Waters Acquity^® PDA @ 254 nm
Instrument	Waters ACQUITY UPLC H-Class

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