Benzodiazepines on Raptor Biphenyl by LC-MS

LC_CF0563

	Peaks	t_R (min)	SIM
1.	Bromazepam	1.23	317.95
2.	Flurazepam	1.36	388.10
3.	Oxazepam	2.05	287.05
4.	Nitrazepam	2.16	282.05
5.	Lorazepam	2.20	321.00
6.	Alprazolam	2.55	316.00

	Peaks	t_R (min)	SIM
7.	Clonazepam	2.61	309.05
8.	Triazolam	2.71	343.05
9.	Temazepam	3.02	301.05
10.	Flunitrazepam	3.25	314.05
11.	Clobazam	3.28	301.05
12.	Diazepam	3.54	285.05

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Temp.:

40 °C

Standard/Sample

Diluent:

Water:acetonitrile (70:30)

Conc.:

5 µg/mL

Inj. Vol.:

1 µL

Mobile Phase

A:

2 mM Ammonium formate + 0.2% formic acid in water

B:

0.2% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	70	30
3.0	0.5	60	40
3.5	0.5	50	50
3.6	0.5	5	95
4.0	0.5	5	95
4.1	0.5	70	30
5.0	0.5	70	30

Detector	Shimadzu LCMS-2010EV
Acquisition Type:	Scan/SIM
Probe Voltage:	1.5 kV
Nebulizing Gas Flow:	1.5 L/min
Drying Gas Pressure:	0.1 MPa
CDL Temp.:	300 °C
Heat Block:	300 °C
Interface:	ESI+
Instrument	Shimadzu UFLCXR

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