Pain Panel in Urine on Raptor Biphenyl (50 x 3.0 mm) by LC-MS/MS

	Peaks	t_R (min)	Precursor ion	Product ion 1	Product ion 2
1.	Morphine*	1.34	286.2	152.3	165.3
2.	Oxymorphone	1.40	302.1	227.3	198.2
3.	Hydromorphone*	1.52	286.1	185.3	128.2
4.	Amphetamine	1.62	136.0	91.3	119.2
5.	Methamphetamine	1.84	150.0	91.2	119.3
6.	Codeine*	1.91	300.2	165.4	153.2
7.	Oxycodone	2.02	316.1	241.3	256.4
8.	Hydrocodone*	2.06	300.1	199.3	128.3
9.	Norbuprenorphine	2.59	414.1	83.4	101.0

	Peaks	t_R (min)	Precursor ion	Product ion 1	Product ion 2
10.	Meprobamate	2.61	219.0	158.4	97.2
11.	Fentanyl	2.70	337.2	188.4	105.2
12.	Buprenorphine	2.70	468.3	396.4	414.5
13.	Flurazepam	2.73	388.2	315.2	288.3
14.	Sufentanil	2.77	387.2	238.5	111.3
15.	Methadone	2.86	310.2	265.3	105.3
16.	Carisoprodol	2.87	261.2	176.3	158.1
17.	Lorazepam	3.03	321.0	275.4	303.1
18.	Diazepam	3.31	285.1	193.2	153.9

*An extracted ion chromatogram (XIC) of the isobars is presented in the inset.

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Temp.:

30 °C

Standard/Sample

Diluent:

Urine:mobile phase A:mobile phase B (17:76:7)

Conc.:

10-100 ng/mL

Inj. Vol.:

10 µL

Mobile Phase

Water + 0.1% formic acid

Methanol + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	90	10
1.50	0.6	55	45
2.50	0.6	0	100
3.70	0.6	0	100
3.71	0.6	90	10
5.00	0.6	90	10

Detector	AB SCIEX API 4000 MS/MS
Ion Source:	TurboIonSpray^®
Ion Mode:	ESI+
Instrument	API LC-MS/MS
Notes	Lorazepam was prepared at 100 ng/mL; all other analytes are 10 ng/mL.

Download PDF