Therapeutic Drugs in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	Morphine*	1.05	286.2	152.3	165.3
2.	Oxymorphone	1.10	302.1	227.3	198.2
3.	Pregabalin	1.13	160.2	97.1	124.1
4.	Hydromorphone*	1.18	286.1	185.3	128.2
5.	Gabapentin	1.32	172.2	137.0	95.1
6.	Codeine-d3 (IS)	1.51	303.2	181.0	-
7.	Codeine*	1.51	300.2	153.2	165.4
8.	Oxycodone	1.60	316.1	241.3	256.4
9.	Hydrocodone*	1.64	300.1	199.3	128.3
10.	Tapentadol	1.89	222.2	107.1	121.1
11.	Zopiclone	2.12	389.1	245.2	345.2
12.	7-Aminoclonazepam	2.21	286.0	222.2	250.3
13.	Norbuprenorphine	2.22	414.1	83.4	101.0
14.	Zolpidem	2.36	308.2	235.0	263.1
15.	Citalopram	2.37	325.2	109.2	262.2
16.	Doxepin-d3 (IS)	2.44	283.2	115.1	-

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
17.	Doxepin	2.44	280.1	107.2	235.1
18.	Fentanyl	2.44	337.2	188.4	105.2
19.	Buprenorphine	2.46	468.3	396.4	414.5
20.	7-Aminoflunitrazepam	2.46	284.2	135.2	240.2
21.	Promethazine	2.59	285.2	86.2	71.1
22.	Paroxetine	2.61	330.2	192.1	70.1
23.	EDDP	2.65	279.1	235.1	250.2
24.	Nortriptyline	2.66	264.2	233.1	105.1
25.	Amitriptyline	2.68	278.2	105.1	91.1
26.	Sertraline	2.78	307.1	276.0	160.1
27.	Methadone	2.79	310.2	265.3	105.3
28.	Lorazepam	2.83	321.1	275.1	228.9
29.	Clonazepam	2.90	315.9	270.2	214.1
30.	Flunitrazepam	3.10	314.4	239.1	211.0
31.	Diazepam-d5 (IS)	3.26	290.0	154.1	-
32.	Diazepam	3.27	284.8	193.1	222.2

*An extracted ion chromatogram (XIC) of the isobars is presented in the inset

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Pore Size:

100 Å

Guard Column:

Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)

Temp.:

40 °C

Standard/Sample

Therapeutic drug custom mix

Conc.:

A 50-500 ng/mL standard was prepared in urine. 100 µL of the 50-500 ng/mL urine standard was diluted with 900 µL water prior to analysis (10x dilution).

Inj. Vol.:

20 µL

Mobile Phase

2 mM ammonium formate and 0.1% formic acid in water

2 mM ammonium formate and 0.1% formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	90	10
3.00	0.8	0	100
3.50	0.8	0	100
3.51	0.8	90	10
5.00	0.8	90	10

Detector	ABSCIEX API 4000 MS/MS
Ion Source:	TurboIonSpray^®
Ion Mode:	ESI+
Mode:	Scheduled MRM
Instrument	API LC-MS/MS
Notes	1. Lorazepam was prepared at 500 ng/mL; all other analytes are 50 ng/mL. 2. Doxepin is an isomeric mixture (cis:trans, 15:85) and results in two peaks. The trans form is the primary isomer peak.

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