Norbuprenorphine & Buprenorphine in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Parent Ion	Product Ion 1	Product Ion 2
1.	Norbuprenorphine	0.63	414.1	83.4	101.0
2.	Buprenorphine	0.84	468.3	396.4	414.5

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Temp.:

30 °C

Sample

Conc.:

100 ng/mL standard was prepared in 1 mL urine. The 1 mL urine standard was then diluted with 2 mL of 0.1% formic acid in methanol:water (50:50) prior to analysis.

Inj. Vol.:

10 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.75	45	55
1.0	0.75	0	100
1.5	0.75	0	100
1.51	0.75	45	55
2.0	0.75	45	55

Detector	AB SCIEX API 4000 MS/MS
Ion Source:	TurboIonSpray^®
Ion Mode:	ESI+
Instrument	API LC-MS/MS

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