THC + Metabolites in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	THC-OH-d3 (IS)	1.94	334.35	196.26	-
2.	THC-OH	1.94	331.32	193.18	105.11
3.	THC-COOH-d3 (IS)	2.09	348.35	196.24	-
4.	THC-COOH	2.09	345.36	193.18	119.16
5.	Δ9-THC-d3 (IS)	2.32	318.42	196.25	-
6.	Δ9-THC	2.33	315.32	193.18	135.16

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)

Temp.:

40 °C

Standard/Sample

Custom

Conc.:

A 50 ng/mL standard was prepared in urine. 1 mL of the 50 ng/mL urine standard was diluted with 2 mL of 0.1% formic acid in 50:50 water:methanol prior to analysis.

Inj. Vol.:

2 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.700	40	60
2.5	0.700	0	100
3.0	0.700	0	100
3.01	0.700	40	60
4.0	0.700	40	60

Detector	Waters Xevo TQ-S
Ion Source:	Waters Zspray™ ESI
Ion Mode:	ESI+
Instrument	Waters

Download PDF