Synthetic Cannabinoids in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	Pravadoline	2.11	379.29	135.04	114.16
2.	AM2233	2.38	459.25	112.20	98.15
3.	JWH-200-d5 (IS)	2.43	390.34	155.07	-
4.	JWH-200	2.45	385.28	155.07	114.16
5.	WIN 55, 212	3.41	427.29	155.07	127.14
6.	RCS-4	4.16	322.27	135.12	77.09
7.	JWH-015-d7 (IS)	4.27	335.28	155.07	-
8.	JWH-250	4.27	336.28	121.12	91.07
9.	JWH-015	4.29	328.26	155.07	127.13

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
10.	AM2201	4.30	360.26	155.07	127.14
11.	JWH-203	4.39	340.23	188.18	125.09
12.	JWH-073	4.42	328.26	155.07	127.13
13.	JWH-018-d9 (IS)	4.55	351.34	155.07	-
14.	JWH-018	4.57	342.27	155.08	127.11
15.	JWH-081	4.64	372.28	185.12	157.09
16.	JWH-122	4.69	356.29	169.12	141.11
17.	JWH-019	4.70	356.29	155.07	127.10
18.	JWH-210	4.84	370.31	183.12	153.26

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)

Temp.:

30 °C

Standard/Sample

Custom

Conc.:

A 5 ng/mL standard was prepared in urine. 1 mL of the 5 ng/mL urine standard was diluted with 2 mL of 0.1% formic acid in 50:50 water:methanol prior to analysis.

Inj. Vol.:

2 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.600	75	25
1.0	0.600	75	25
5.0	0.600	5	95
5.5	0.600	5	95
5.51	0.600	75	25
7.0	0.600	75	25

Detector	Waters Xevo TQ-S
Ion Source:	Waters Zspray™ ESI
Ion Mode:	ESI+
Mode:	MRM
Instrument	Waters
Notes	Chromatogram collected using scheduled MRMs.

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