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Peaks | tR (min) | Q1 | Q3 Quantifier | Q3 Qualifier | |
---|---|---|---|---|---|
1. | JWH-073 N-butanoic acid | 1.27 | 358.27 | 155.08 | 127.11 |
2. | JWH-073 4-hydroxybutyl | 1.31 | 344.24 | 155.09 | 127.09 |
3. | JWH-018 N-pentanoic acid | 1.39 | 372.18 | 155.08 | 127.14 |
4. | JWH-018 5-hydroxypentyl-d5 (IS) | 1.44 | 363.50 | 155.08 | - |
5. | JWH-018 5-hydroxypentyl | 1.46 | 358.27 | 155.08 | 127.11 |
6. | JWH-073 6-hydroxyindole | 1.72 | 344.24 | 155.09 | 127.09 |
7. | JWH-073 5-hydroxyindole-d7 (IS) | 1.78 | 351.21 | 155.07 | - |
Peaks | tR (min) | Q1 | Q3 Quantifier | Q3 Qualifier | |
---|---|---|---|---|---|
8. | JWH-073 5-hydroxyindole | 1.80 | 344.24 | 155.09 | 127.09 |
9. | JWH-073 7-hydroxyindole | 1.94 | 344.24 | 155.09 | 127.09 |
10. | JWH-018 6-hydroxyindole | 1.96 | 358.27 | 155.08 | 127.11 |
11. | JWH-018 5-hydroxyindole | 2.04 | 358.27 | 155.08 | 127.11 |
12. | JWH-018 7-hydroxyindole | 2.18 | 358.27 | 155.08 | 127.11 |
13. | JWH-073 4-hydroxyindole | 2.91 | 344.24 | 155.09 | 127.09 |
14. | JWH-018 4-hydroxyindole | 3.16 | 358.27 | 155.08 | 127.11 |
Column | Raptor Biphenyl (cat.# 9309A5E) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 3.0 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253) | ||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||
Standard/Sample | Custom | ||||||||||||||||||||||||
Conc.: | A 10 ng/mL standard was prepared in urine. 100 μL of the 10 ng/mL urine standard was diluted with 300 μL of 50:50 water:acetonitrile prior to analysis. | ||||||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||||||
B: | 0.1% Formic acid in acetonitrile | ||||||||||||||||||||||||
|
Detector | Waters Xevo TQ-S |
---|---|
Ion Source: | Waters Zspray™ ESI |
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | Waters |
Notes |
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