Synthetic Cannabinoid Metabolites in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	JWH-073 N-butanoic acid	1.27	358.27	155.08	127.11
2.	JWH-073 4-hydroxybutyl	1.31	344.24	155.09	127.09
3.	JWH-018 N-pentanoic acid	1.39	372.18	155.08	127.14
4.	JWH-018 5-hydroxypentyl-d5 (IS)	1.44	363.50	155.08	-
5.	JWH-018 5-hydroxypentyl	1.46	358.27	155.08	127.11
6.	JWH-073 6-hydroxyindole	1.72	344.24	155.09	127.09
7.	JWH-073 5-hydroxyindole-d7 (IS)	1.78	351.21	155.07	-

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
8.	JWH-073 5-hydroxyindole	1.80	344.24	155.09	127.09
9.	JWH-073 7-hydroxyindole	1.94	344.24	155.09	127.09
10.	JWH-018 6-hydroxyindole	1.96	358.27	155.08	127.11
11.	JWH-018 5-hydroxyindole	2.04	358.27	155.08	127.11
12.	JWH-018 7-hydroxyindole	2.18	358.27	155.08	127.11
13.	JWH-073 4-hydroxyindole	2.91	344.24	155.09	127.09
14.	JWH-018 4-hydroxyindole	3.16	358.27	155.08	127.11

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)

Temp.:

30 °C

Standard/Sample

Custom

Conc.:

A 10 ng/mL standard was prepared in urine. 100 μL of the 10 ng/mL urine standard was diluted with 300 μL of 50:50 water:acetonitrile prior to analysis.

Inj. Vol.:

2 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.600	50	50
4.00	0.600	15	85
4.50	0.600	15	85
4.51	0.600	50	50
6.00	0.600	50	50

Detector	Waters Xevo TQ-S
Ion Source:	Waters Zspray™ ESI
Ion Mode:	ESI+
Mode:	MRM
Instrument	Waters
Notes

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