Vitamin D Metabolites on Raptor ARC-18 by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Q1	Q3
1.	1,25-Dihydroxyvitamin D3	0.88	200	399.4	381.5
2.	25-Hydroxyvitamin D3	1.33	200	401.5	383.5
3.	25-Hydroxyvitamin D2	1.41	200	413.5	395.5
4.	Vitamin D2	3.47	200	397.5	379.6
5.	Vitamin D3	3.53	200	385.5	367.5

Column

Raptor ARC-18 (cat.# 9314A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

40 °C

Sample

Diluent:

Methanol

Conc.:

200 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

0.1% Formic acid + 5 mM ammonium formate in water

0.1% Formic acid + 5 mM ammonium formate in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	10	90
4.00	0.5	0	100
4.01	0.5	10	90
6.00	0.5	10	90

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