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Peaks | tR (min) | Conc. (ng/mL) | Q1 | Q3 | |
---|---|---|---|---|---|
1. | 1,25-Dihydroxyvitamin D3 | 0.88 | 200 | 399.4 | 381.5 |
2. | 25-Hydroxyvitamin D3 | 1.33 | 200 | 401.5 | 383.5 |
3. | 25-Hydroxyvitamin D2 | 1.41 | 200 | 413.5 | 395.5 |
4. | Vitamin D2 | 3.47 | 200 | 397.5 | 379.6 |
5. | Vitamin D3 | 3.53 | 200 | 385.5 | 367.5 |
Column | Raptor ARC-18 (cat.# 9314A12) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | Methanol | ||||||||||||||||||||
Conc.: | 200 ng/mL | ||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% Formic acid + 5 mM ammonium formate in water | ||||||||||||||||||||
B: | 0.1% Formic acid + 5 mM ammonium formate in methanol | ||||||||||||||||||||
|
Detector | ABSciex API 4000 |
---|---|
Ion Source: | TurboIonSpray® |
Ion Mode: | ESI+ |
Instrument | Shimadzu UFLCXR |
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