Naltrexone and 6-β-Naltrexol in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Naltrexone	0.90	342.4	270.1	267.1
2.	6-β-Naltrexol	0.98	344.4	308.0	254.1

Column

Raptor Biphenyl (cat.# 9309552)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Temp.:

30 °C

Sample

Conc.:

Prepared 500 ng/mL in human urine. Diluted 5x in mobile phase A. Final conc. = 100 ng/mL

Inj. Vol.:

10 µL

Mobile Phase

Water + 0.1% formic acid + 2 mM ammonium formate

Water:methanol (5:95) + 0.1% formic acid + 2 mM ammonium formate

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	70	30
1.50	0.5	0	100
1.51	0.5	70	30
3.00	0.5	70	30

Max Pressure:

110 bar

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