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Naltrexone and 6-β-Naltrexol in Urine on Raptor Biphenyl by LC-MS/MS

LC_CF0588
PeakstR (min)Precursor IonProduct Ion 1Product Ion 2
1.Naltrexone0.90342.4270.1267.1
2.6-β-Naltrexol0.98344.4308.0254.1
ColumnRaptor Biphenyl (cat.# 9309552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Temp.:30 °C
Sample
Conc.: Prepared 500 ng/mL in human urine. Diluted 5x in mobile phase A. Final conc. = 100 ng/mL
Inj. Vol.:10 µL
Mobile Phase
A:Water + 0.1% formic acid + 2 mM ammonium formate
B:Water:methanol (5:95) + 0.1% formic acid + 2 mM ammonium formate
Time (min)Flow (mL/min)%A%B
0.000.57030
1.500.50100
1.510.57030
3.000.57030
Max Pressure:110 bar
DetectorMS/MS
Ion Mode:ESI+
InstrumentHPLC