Thyroid Hormones on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	3,3',5-Triiodo-L-thyronine (T3)	1.05	651.84	605.80
2.	3,3',5'-Triiodo-L-thyronine (rT3)	1.28	651.84	605.80
3.	L-Thyroxine (T4)	1.48	777.71	731.72

Column

Raptor Biphenyl (cat.# 9309512)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

5 µm

Temp.:

40 °C

Standard/Sample

Diluent:

Water:methanol (35:65)

Conc.:

1 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	35	65
2.00	0.4	20	80
2.01	0.4	35	65
3.50	0.4	35	65

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