Macrolide Antibiotics on Raptor C18 by LC-MS/MS

LC_CF0600

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	Spiramycin	1.32	844.21	174.28
2.	Azithromycin	1.33	750.14	591.83
3.	Dirithromycin	1.52	836.26	158.23
4.	Tilmicosin	1.58	870.23	174.29
5.	Erythromycin	1.87	735.09	158.23
6.	Tylosin	1.98	917.26	174.28
7.	Clarithromycin	2.22	749.11	158.22
8.	Roxithromycin	2.26	838.21	158.23
9.	Josamycin	2.40	829.15	109.18

Column

Raptor C18 (cat.# 9304512)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

Water:acetonitrile (50:50)

Conc.:

100 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	80	20
3.00	0.4	35	65
3.01	0.4	80	20
4.50	0.4	80	20

Detector	MS/MS
Ion Mode:	ESI+
Instrument	UHPLC

Download PDF