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Macrolide Antibiotics on Raptor C18 by LC-MS/MS

LC_CF0600
PeakstR (min)Precursor IonProduct Ion
1.Spiramycin1.32844.21174.28
2.Azithromycin1.33750.14591.83
3.Dirithromycin1.52836.26158.23
4.Tilmicosin1.58870.23174.29
5.Erythromycin1.87735.09158.23
6.Tylosin1.98917.26174.28
7.Clarithromycin2.22749.11158.22
8.Roxithromycin2.26838.21158.23
9.Josamycin2.40829.15109.18
ColumnRaptor C18 (cat.# 9304512)
Dimensions:100 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:Water:acetonitrile (50:50)
Conc.:100 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.48020
3.000.43565
3.010.48020
4.500.48020
DetectorMS/MS
Ion Mode:ESI+
InstrumentUHPLC