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Peaks | tR (min) | Precursor Ion | Product Ion | |
---|---|---|---|---|
1. | Tri-iodothyronine (T3) | 1.04 | 652.07 | 606.10 |
2. | Reverse tri-iodothyronine (rT3) | 1.25 | 652.07 | 606.10 |
3. | Thyroxin (T4) | 1.43 | 778.03 | 731.97 |
4. | Thyroxin-13C6 | 1.43 | 784.09 | 738.04 |
Column | Raptor Biphenyl (cat.# 9309A12) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252) | ||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | 30:70 Water:methanol | ||||||||||||||||||||
Conc.: | 20 pg/mL | ||||||||||||||||||||
Inj. Vol.: | 10 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||
B: | 0.1% Formic acid in methanol | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | HPLC |
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