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Thyroid Hormone Analysis in 4% Human Albumin in PBS on Raptor Biphenyl by LC-MS/MS

LC_CF0605
PeakstR (min)Precursor IonProduct Ion
1.Tri-iodothyronine (T3)1.04652.07606.10
2.Reverse tri-iodothyronine (rT3)1.25652.07606.10
3.Thyroxin (T4)1.43778.03731.97
4.Thyroxin-13C61.43784.09738.04
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:40 °C
Sample
Diluent:30:70 Water:methanol
Conc.:20 pg/mL
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.43070
2.000.42080
2.010.43070
3.500.43070
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentHPLC