Thyroid Hormone Analysis in 4% Human Albumin in PBS on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	Tri-iodothyronine (T3)	1.04	652.07	606.10
2.	Reverse tri-iodothyronine (rT3)	1.25	652.07	606.10
3.	Thyroxin (T4)	1.43	778.03	731.97
4.	Thyroxin-13C6	1.43	784.09	738.04

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

40 °C

Sample

Diluent:

30:70 Water:methanol

Conc.:

20 pg/mL

Inj. Vol.:

10 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	30	70
2.00	0.4	20	80
2.01	0.4	30	70
3.50	0.4	30	70

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