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Coenzyme Q10 in Serum on Raptor Biphenyl by LC-MS/MS

LC_CF0611
PeakstR (min)Precursor IonProduct Ion
1.Ubiquinol-100.98882.82197.18
2.Ubiquinone-10-D91.92872.89206.16
3.Ubiquinone-101.95863.86197.12
ColumnRaptor Biphenyl (cat.# 9309552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)
Temp.:40 °C
Standard/Sample
Diluent:See sample preparation
Conc.: Endogenous levels in serum
Inj. Vol.:5 µL
Mobile Phase
A:0.1% formic acid, 5 mM ammonium formate in 50:50 water:acetonitrile
B:0.1% formic acid, 5 mM ammonium formate in methanol
Time (min)Flow (mL/min)%A%B
0.000.6595
2.500.60100
2.510.6595
4.000.6595
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
Sample PreparationA 150 μL aliquot of serum was mixed with 2.5 μL of ubiquinone-10-D9 (10 μg/mL in methanol) and 850 μL of 1-propanol. Stirred to mix for 2 minutes and then centrifuged at 4000 rpm for 5 minutes. Transferred a portion of extract to HPLC vial for analysis.