Coenzyme Q10 in Serum on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	Ubiquinol-10	0.98	882.82	197.18
2.	Ubiquinone-10-D9	1.92	872.89	206.16
3.	Ubiquinone-10	1.95	863.86	197.12

Column

Raptor Biphenyl (cat.# 9309552)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)

Temp.:

40 °C

Standard/Sample

Diluent:

See sample preparation

Conc.:

Endogenous levels in serum

Inj. Vol.:

5 µL

Mobile Phase

0.1% formic acid, 5 mM ammonium formate in 50:50 water:acetonitrile

0.1% formic acid, 5 mM ammonium formate in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	5	95
2.50	0.6	0	100
2.51	0.6	5	95
4.00	0.6	5	95

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	A 150 μL aliquot of serum was mixed with 2.5 μL of ubiquinone-10-D9 (10 μg/mL in methanol) and 850 μL of 1-propanol. Stirred to mix for 2 minutes and then centrifuged at 4000 rpm for 5 minutes. Transferred a portion of extract to HPLC vial for analysis.

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