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Peaks | tR (min) | Precursor Ion | Product Ion | |
---|---|---|---|---|
1. | Ubiquinol-10 | 0.98 | 882.82 | 197.18 |
2. | Ubiquinone-10-D9 | 1.92 | 872.89 | 206.16 |
3. | Ubiquinone-10 | 1.95 | 863.86 | 197.12 |
Column | Raptor Biphenyl (cat.# 9309552) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 5 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252) | ||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | See sample preparation | ||||||||||||||||||||
Conc.: | Endogenous levels in serum | ||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% formic acid, 5 mM ammonium formate in 50:50 water:acetonitrile | ||||||||||||||||||||
B: | 0.1% formic acid, 5 mM ammonium formate in methanol | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
Sample Preparation | A 150 μL aliquot of serum was mixed with 2.5 μL of ubiquinone-10-D9 (10 μg/mL in methanol) and 850 μL of 1-propanol. Stirred to mix for 2 minutes and then centrifuged at 4000 rpm for 5 minutes. Transferred a portion of extract to HPLC vial for analysis. |
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