Synthetic Cannabinoids in Urine by LC-MS/MS on Raptor Biphenyl

	Peaks	t_R (min)	Precursor Ion	Quantifier Product Ion	Qualifier Product
1.	Pravadoline	2.15	379.29	135.04	114.16
2.	AM2233	2.44	459.25	112.2	98.15
3.	JWH-200-d5	2.47	390.34	155.07	NA
4.	JWH-200	2.48	385.28	155.07	114.16
5.	WIN 55, 212	3.34	427.29	155.07	127.14
6.	RCS-4	4.15	322.27	135.12	77.09
7.	XLR-11	4.21	330.25	232.17	125.1
8.	JWH-015-d7	4.27	335.28	155.07	NA
9.	JWH-250	4.27	336.28	121.12	91.07
10.	JWH-015	4.29	328.26	155.07	127.13

	Peaks	t_R (min)	Precursor Ion	Quantifier Product Ion	Qualifier Product
11.	AM2201	4.30	360.26	155.07	127.14
12.	JWH-203	4.39	340.23	188.18	125.09
13.	JWH-073	4.42	328.26	155.07	127.13
14.	UR-144	4.44	312.32	214.17	125.1
15.	JWH-018-d9	4.55	351.34	155.07	NA
16.	JWH-018	4.57	342.27	155.08	127.11
17.	JWH-081	4.64	372.28	185.12	157.09
18.	JWH-122	4.69	356.29	169.12	141.11
19.	JWH-019	4.70	356.29	155.07	127.1
20.	JWH-210	4.84	370.31	183.12	153.26

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5.0 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)

Standard/Sample

Conc.:

5 ng/mL standard was prepared in urine and diluted 3x with 50:50 methanol:water

Inj. Vol.:

2 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	75	25
1.00	0.6	75	25
5.00	0.6	5	95
5.50	0.6	5	95
5.51	0.6	75	25
7.00	0.6	75	25

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