Anti-Anxiety Drug Panel on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	7-Aminonitrazepam	1.22	252.2	121.1	94.2
2.	Norketamine	1.34	224.1	125.0	89.1
3.	Zolpidem Phenyl-4-carboxylic acid	1.38	338.2	265.1	219.0
4.	Ketamine	1.45	238.1	125.1	89.1
5.	Zopiclone	1.54	389.2	245.0	217.2
6.	Meprobamate	1.59	219.1	158.2	97.0
7.	7-Aminoclonazepam	1.60	286.1	121.2	250.1
8.	Zolpidem	1.72	308.2	235.2	218.9
9.	Diphenhydramine	1.74	256.1	167.0	152.1
10.	Flurazepam	1.83	388.2	315.2	183.0
11.	Buspirone	1.86	386.3	122.1	95.0
12.	7-Aminoflunitrazepam	1.87	284.1	135.0	227.1
13.	Chlordiazepoxide	1.88	300.1	227.0	282.0
14.	Midazolam	1.92	326.2	291.1	248.9
15.	Cyclobenzaprine	1.94	276.2	215.0	189.0
16.	Carisoprodol	1.96	261.1	176.0	62.0
17.	α-Hydroxymidazolam	2.03	342.2	324.1	202.9
18.	Metaxalone	2.14	222.1	161.1	77.1
19.	Lorazepam	2.20	321.1	275.0	229.0

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
20.	N-Desmethylflunitrazepam	2.23	300.1	254.1	198.1
21.	Oxazepam	2.28	287.1	241.2	268.8
22.	Clonazepam	2.30	316.1	270.0	214.1
23.	Desalkylflurazepam	2.31	289.0	140.1	104.0
24.	Nitrazepam	2.32	282.1	235.9	180.1
25.	α-Hydroxytriazolam	2.34	359.1	330.9	175.9
26.	2-Hydroxyethylflurazepam	2.35	333.1	211.1	109.0
27.	α-Hydroxyalprazolam	2.42	325.1	297.0	216.2
28.	Methaqualone	2.44	251.1	132.2	91.1
29.	Nordiazepam	2.44	271.0	139.9	208.0
30.	Phenazepam	2.44	349.1	206.2	179.0
31.	Zaleplon	2.53	306.1	264.0	236.0
32.	Triazolam	2.58	343.1	307.9	315.0
33.	Flunitrazepam	2.59	314.2	267.9	239.1
34.	Estazolam	2.61	295.1	266.9	205.1
35.	Temazepam	2.61	301.1	255.1	282.9
36.	Alprazolam	2.68	309.1	280.9	204.9
37.	Diazepam	2.85	285.1	153.9	192.9
38.	Prazepam	3.14	325.2	271.0	139.9

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

30 °C

Standard/Sample

Diluent:

Water

Conc.:

10 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

Water + 0.1% formic acid

Methanol + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	70	30
1.50	0.6	20	80
3.00	0.6	5	95
3.50	0.6	5	95
3.51	0.6	70	30
5.50	0.6	70	30

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