Barbiturate Drug Panel on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Phenobarbital	1.97	230.9	187.8	85.0
2.	Butalbital	2.21	223.0	180.0	84.9
3.	Amobarbital	2.95	225.0	182.0	84.8
4.	Pentobarbital	3.04	225.0	182.0	84.9
5.	Secobarbital	3.69	237.0	193.9	84.8

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

30 °C

Standard/Sample

Diluent:

Water

Conc.:

500 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

Water + 0.1% formic acid

Methanol + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	55	45
4.00	0.6	45	55
4.01	0.6	55	45
6.00	0.6	55	45

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