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Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|
1. | Phenobarbital | 1.97 | 230.9 | 187.8 | 85.0 |
2. | Butalbital | 2.21 | 223.0 | 180.0 | 84.9 |
3. | Amobarbital | 2.95 | 225.0 | 182.0 | 84.8 |
4. | Pentobarbital | 3.04 | 225.0 | 182.0 | 84.9 |
5. | Secobarbital | 3.69 | 237.0 | 193.9 | 84.8 |
Column | Raptor Biphenyl (cat.# 9309A12) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252) | ||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | Water | ||||||||||||||||||||
Conc.: | 500 ng/mL | ||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | Water + 0.1% formic acid | ||||||||||||||||||||
B: | Methanol + 0.1% formic acid | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI- |
Mode: | MRM |
Instrument | UHPLC |
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