Barbiturate Drug Panel on Raptor C18 by LC-MS/MS

LC_CF0619

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Phenobarbital	1.39	230.9	187.8	85.0
2.	Butalbital	2.01	223.0	180.0	84.9
3.	Pentobarbital	2.91	225.0	182.0	84.9
4.	Amobarbital	3.03	225.0	182.0	84.8
5.	Secobarbital	3.71	237.0	193.9	84.8

Column

Raptor C18 (cat.# 9304A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

50 °C

Standard/Sample

Diluent:

Water

Conc.:

500 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

Water + 0.1% formic acid

B:

Acetonitrile + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	80	20
4.00	0.6	72	28
4.01	0.6	80	20
6.00	0.6	80	20

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	UHPLC

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