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Barbiturate Drug Panel on Raptor C18 by LC-MS/MS

LC_CF0619
PeakstR (min)Precursor IonProduct IonProduct Ion
1.Phenobarbital1.39230.9187.885.0
2.Butalbital2.01223.0180.084.9
3.Pentobarbital2.91225.0182.084.9
4.Amobarbital3.03225.0182.084.8
5.Secobarbital3.71237.0193.984.8
ColumnRaptor C18 (cat.# 9304A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:50 °C
Standard/Sample
Diluent:Water
Conc.:500 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:Water + 0.1% formic acid
B:Acetonitrile + 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.68020
4.000.67228
4.010.68020
6.000.68020
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC