Multiclass Drug Panel on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	trans-3-Hydroxycotinine	1.32	25	193.1	80.0	134.0
2.	Norcotinine	1.33	25	163.1	80.1	118.1
3.	Nicotine	1.40	25	163.2	130.1	117.1
4.	BZP	1.47	25	177.2	91.1	65.1
5.	Anabasine	1.53	25	163.2	120.1	80.1
6.	Noroxymorphone	1.78	25	288.1	270.1	212.9
7.	Lisdexamfetamine	1.79	25	264.2	84.1	91.0
8.	Phenelzine sulfate	1.90	25	137.2	105.1	77.1
9.	Acetaminophen	1.93	100	152.2	93.0	65.0
10.	Pregabalin	2.21	25	160.2	142.1	55.0
11.	Cotinine	2.27	25	177.2	80.0	98.1
12.	Morphine	2.27	25	286.2	152.1	165.0
13.	(+)-Ephedrine	2.37	25	166.2	148.1	115.0
14.	Oxymorphone	2.38	25	302.1	227.2	198.2
15.	Hydromorphone	2.61	25	286.2	184.9	156.9
16.	Amphetamine	2.63	25	136.2	91.0	65.1
17.	DMAA	2.65	25	116.1	57.1	99.2
18.	Gabapentin	2.71	25	172.2	154.0	137.1
19.	Methylephedrine	2.83	25	180.2	162.0	115.1
20.	Levetiracetam	2.84	25	171.1	125.9	69.2
21.	Desmethylolanzapine	2.94	25	299.1	256.1	198.0
22.	Norcodeine	2.96	25	286.1	151.9	115.0
23.	Olanzapine	3.12	25	313.2	256.1	198.1
24.	Methamphetamine	3.13	25	150.3	91.1	119.0
25.	MDA	3.18	25	180.1	163.1	105.2
26.	(-)-Naloxone	3.27	25	328.3	310.1	212.3
27.	Phentermine	3.29	25	150.2	91.1	133.1
28.	Noroxycodone	3.32	25	302.1	227.0	197.9
29.	Methylone	3.36	25	208.1	160.1	132.1
30.	O-Desmethyl-cis-tramadol	3.37	25	250.1	58.0	42.0
31.	(-)-Dihydrocodeine	3.38	25	302.2	199.0	128.2
32.	Norhydrocodone	3.43	25	286.1	199.0	128.2
33.	Codeine	3.43	25	300.2	152.0	165.1
34.	6-Acetylmorphine	3.47	25	328.2	165.0	211.0
35.	MDMA	3.60	25	194.2	163.0	135.1
36.	Desomorphine	3.61	25	272.1	152.1	165.0
37.	Oxycodone	3.64	25	316.2	298.0	169.0
38.	Naltrexone	3.71	25	342.2	324.0	267.0
39.	Levamisole	3.75	25	205.1	178.0	91.1
40.	Hydrocodone	3.76	25	300.1	199.0	128.0
41.	Diethylpropion	3.80	25	206.2	105.1	100.1
42.	Mephedrone	3.80	25	178.2	145.0	160.1
43.	2-Oxo-3-hydroxy-LSD	3.82	25	356.3	237.0	222.0
44.	o-Desmethylvenlafaxine	3.84	25	264.1	58.1	107.0
45.	6-β-Naltrexol	3.91	25	344.3	326.1	308.1
46.	Tapentadol-β-D-glucuronide	3.91	25	398.3	222.1	107.0
47.	7-Aminonitrazepam	3.95	25	252.2	121.1	94.2
48.	MDEA	4.00	25	208.1	163.0	105.2
49.	m-Hydroxybenzoylecgonine	4.02	25	306.1	168.1	121.0
50.	Lamotrigine	4.09	25	256.0	211.1	145.0
51.	Ritalinic acid	4.11	25	220.1	84.1	56.2
52.	N-Desmethyltapentadol	4.16	25	208.1	121.2	107.1
53.	Norbuprenorphine glucuronide	4.18	25	590.5	414.3	165.0
54.	Zonisamide	4.23	25	212.9	132.1	77.1
55.	mCPP	4.26	25	197.1	154.0	118.1
56.	Norketamine	4.28	25	224.1	125.0	89.1
57.	Nalbuphine	4.33	25	358.2	340.1	115.0
58.	Norfentanyl	4.33	25	233.1	84.1	55.0
59.	(+/-)-Hydroxybupropion	4.35	25	256.0	130.2	166.0
60.	Tapentadol	4.37	25	222.1	107.1	121.0
61.	Lacosamide	4.47	25	251.5	91.1	108.1
62.	Dextrorphan	4.50	25	258.2	157.0	201.2
63.	Levorphanol	4.50	25	258.2	156.8	199.2
64.	7-Hydroxyquetiapine	4.51	25	400.3	269.0	208.0
65.	Molindone	4.51	25	277.2	100.1	70.2
66.	Tramadol	4.52	25	264.2	58.0	77.1
67.	Zolpidem phenyl-4-carboxylic acid	4.60	25	338.2	265.1	219.0
68.	Felbamate	4.62	25	239.1	117.1	177.8
69.	N-Desmethyl-cis-tramadol	4.65	25	250.2	44.0	232.1
70.	Ketamine	4.66	25	238.1	125.1	89.1
71.	Benzoylecgonine	4.67	25	290.1	168.1	77.1
72.	Methylphenidate	4.68	25	234.1	84.1	56.1
73.	Normeperidine	4.76	25	234.1	160.2	91.0
74.	Meprobamate	4.79	25	219.1	158.2	97.0
75.	Meperidine	4.80	25	248.1	220.1	174.1
76.	Bupropion	4.81	25	240.0	184.1	130.2
77.	Heroin	4.85	25	370.4	328.2	164.9
78.	Cocaine	5.01	25	304.2	182.0	77.1
79.	7-Aminoclonazepam	5.02	25	286.1	121.2	250.1
80.	Zopiclone	5.04	25	389.2	245.0	217.2
81.	N-Desmethylmirtazapine	5.10	25	252.1	195.1	209.2
82.	Norcocaine	5.11	25	290.1	168.0	136.1
83.	Phenobarbital*	5.11	500	230.9	187.8	85.0
84.	Buprenorphine glucuronide	5.12	25	644.3	468.2	55.1
85.	MDPV	5.15	25	276.2	135.1	126.1
86.	Venlafaxine	5.15	25	278.4	260.4	121.2
87.	Milnacipran	5.24	25	247.2	100.1	129.1
88.	Pentazocine	5.29	25	286.2	218.1	69.2
89.	Mirtazapine	5.31	25	266.1	195.1	72.1
90.	Butalbital*	5.31	500	223.0	180.0	84.9
91.	Norbuprenorphine	5.35	50	414.3	152.2	165.2
92.	LSD	5.39	25	324.3	223.1	208.0
93.	Butorphanol	5.43	25	328.3	310.3	131.2
94.	Fosphenytoin	5.43	25	363.1	265.9	207.8
95.	Topiramate	5.44	25	340.2	264.2	223.9
96.	Cocaethylene	5.48	25	318.2	196.1	82.0
97.	Clozapine	5.48	25	328.2	271.1	193.1
98.	9-Hydroxyrisperidone	5.51	25	427.3	207.1	110.2
99.	Acetyl fentanyl	5.58	25	323.3	188.0	105.0
100.	Didesmethyl citalopram HCl	5.73	25	297.1	261.9	109.1
101.	7-Aminoflunitrazepam	5.75	25	284.1	135.0	227.1
102.	Amobarbital*	5.75	500	225.0	182.0	84.8
103.	Zolpidem	5.76	25	308.2	235.2	218.9
104.	N-Desmethylcitalopram	5.77	25	311.1	262.1	109.1
105.	Isocarboxazid	5.77	25	232.0	91.1	65.2
106.	Pentobarbital*	5.79	500	225.0	182.0	84.9
107.	Escitalopram	5.80	25	325.3	109.1	261.9
108.	Citalopram	5.80	25	325.2	109.1	262.0
109.	Diphenhydramine	5.80	25	256.1	167.0	152.1
110.	Desmethyldoxepin	5.88	25	266.1	107.1	115.0
111.	Ezogabine	5.91	25	304.0	230.1	109.1
112.	Doxepin	5.96	25	280.1	107.2	77.1
113.	Haloperidol	5.96	25	377.2	123.0	95.1
114.	Fluvoxamine	5.97	25	319.3	71.1	257.8
115.	Trazodone	5.99	25	372.3	176.1	148.0
116.	Norfluoxetine	6.01	25	296.3	134.3	104.9
117.	Fentanyl	6.02	25	337.3	188.0	105.1
118.	Oxcarbazepine	6.02	25	253.1	180.0	208.1
119.	Risperidone	6.03	25	411.2	191.0	110.1
120.	Buprenorphine	6.04	50	468.3	55.1	414.2
121.	Fluoxetine	6.05	25	310.1	148.0	115.1
122.	Secobarbital*	6.08	500	237.0	193.9	84.8
123.	Phenytoin	6.12	25	253.0	103.9	181.7
124.	Carisoprodol	6.13	25	261.1	176.0	62.0
125.	Flurazepam	6.14	25	388.2	315.2	183.0
126.	Chlordiazepoxide	6.14	25	300.1	282.0	227.0
127.	Dextromethorphan	6.16	25	272.2	215.1	170.9
128.	PCP	6.18	25	244.2	86.1	159.1
129.	Tenoxicam	6.18	25	338.1	121.0	95.0
130.	Buspirone	6.20	25	386.3	122.1	95.0
131.	Quetiapine	6.28	25	384.2	253.0	221.2
132.	Norpropoxyphene	6.29	25	326.4	252.0	91.1
133.	Propoxyphene	6.30	25	340.3	265.9	58.1
134.	Asenapine	6.35	25	286.2	165.1	229.1
135.	Ziprasidone	6.40	25	413.2	194.1	130.0
136.	Protriptyline	6.42	25	264.1	191.1	165.2
137.	Promazine	6.42	25	285.1	58.0	86.0
138.	Midazolam	6.43	25	326.2	291.1	248.9
139.	Desipramine	6.43	25	267.1	72.1	193.1
140.	Amoxapine	6.44	25	314.2	271.0	193.1
141.	Sufentanil	6.48	25	387.2	238.1	111.1
142.	Imipramine	6.51	25	281.1	86.2	58.1
143.	Paroxetine	6.51	25	330.2	192.2	70.1
144.	Loxapine	6.52	25	328.1	271.1	193.0
145.	Maprotiline	6.52	25	278.1	250.2	191.0
146.	Duloxetine	6.52	25	298.1	154.1	188.2
147.	Metaxalone	6.52	25	222.1	161.1	77.1
148.	Carbamazepine	6.55	10	237.1	194.1	165.0
149.	Cyclobenzaprine	6.56	25	276.2	215.0	189.0
150.	EDDP	6.57	25	278.2	234.3	249.2
151.	Nortriptyline	6.58	25	264.1	91.1	115.2
152.	Mitragynine	6.59	25	399.3	174.2	159.0
153.	Eslicarbazepine	6.60	25	297.2	194.0	237.1
154.	Amitriptyline	6.65	25	278.1	91.1	202.1
155.	α-Hydroxymidazolam	6.70	25	342.2	324.1	202.9
156.	Trimipramine	6.75	25	295.2	100.2	58.2
157.	N-Desmethylflunitrazepam	6.80	25	300.1	254.1	198.1
158.	Lorazepam	6.82	25	321.1	275.0	229.0
159.	Mesoridazine	6.83	25	387.2	126.1	98.2
160.	Tiagabine	6.89	25	376.2	246.8	111.1
161.	Ketorolac	6.90	25	256.1	104.9	77.0
162.	Chlorpromazine	6.91	25	320.1	58.1	86.1
163.	Loxoprofen*	6.93	200	245.0	83.2	80.9
164.	Clomipramine	6.95	25	315.3	86.0	58.0
165.	Oxazepam	6.96	25	287.1	241.2	268.8
166.	Methadone	6.98	25	310.2	264.9	105.1
167.	Sertraline	6.98	25	306.2	158.9	275.1
168.	Dehydro aripiprazole	7.01	25	446.2	285.0	98.1
169.	Clonazepam	7.01	25	316.1	270.0	214.1
170.	Nitrazepam	7.01	25	282.1	235.9	180.1
171.	Lornoxicam	7.02	100	373.1	121.1	95.0
172.	25I-NBOMe	7.05	25	428.1	121.2	91.1
173.	Salsalate*	7.05	100	256.8	136.8	92.9
174.	Desalkylflurazepam	7.09	25	289.0	140.1	104.0
175.	Tolmetin	7.10	25	258.1	119.1	91.0
176.	α-Hydroxytriazolam	7.12	25	359.1	330.9	175.9
177.	Fluphenazine	7.16	25	438.3	171.1	143.2
178.	2-Hydroxyethylflurazepam	7.16	25	333.1	211.1	109.0
179.	Ketoprofen*	7.17	100	253.0	209.0	-
180.	Naproxen*	7.17	200	229.0	168.9	184.9
181.	EMDP	7.19	25	264.2	235.2	220.3
182.	Aripiprazole	7.20	25	448.2	285.0	176.1
183.	Perphenazine	7.22	25	404.2	171.1	143.2
184.	Etoricoxib	7.24	25	359.2	280.0	279.2
185.	Methaqualone	7.24	25	251.1	91.1	132.2
186.	α-Hydroxyalprazolam	7.25	25	325.1	297.0	216.2
187.	Trifluoperazine	7.28	25	408.2	141.2	113.1
188.	Piroxicam	7.34	25	332.1	95.2	78.1
189.	Ibuprofen*	7.34	200	205.0	160.9	-
190.	Prochlorperazine	7.36	25	374.1	141.0	113.1
191.	Etodolac*	7.36	50	286.0	241.9	212.0
192.	Nordiazepam	7.37	25	271.0	139.9	208.0
193.	Phenazepam	7.39	25	349.1	206.2	179.0
194.	Fenoprofen*	7.40	200	240.9	92.8	-
195.	Zaleplon	7.43	25	306.1	264.0	236.0
196.	Clobazam	7.43	25	301.0	259.1	224.0
197.	Flurbiprofen*	7.48	200	243.0	199.0	196.8
198.	Thiothixene	7.50	25	444.2	221.2	235.0
199.	Flunitrazepam	7.55	25	314.2	267.9	239.1
200.	Meloxicam	7.55	25	352.1	115.0	141.0
201.	Triazolam	7.58	25	343.1	307.9	315.0
202.	Estazolam	7.58	25	295.1	266.9	205.1
203.	Celecoxib	7.60	25	382.2	362.0	300.1
204.	Parecoxib	7.60	25	371.2	234.0	165.1
205.	Temazepam	7.61	25	301.1	255.1	282.9
206.	Sulindac	7.70	25	357.2	233.1	248.0
207.	Alprazolam	7.72	25	309.1	280.9	204.9
208.	Flufenamic acid*	7.75	50	279.9	236.1	176.2
209.	Thioridazine	7.76	25	371.2	126.1	98.1
210.	Diclofenac*	7.78	100	293.9	249.8	-
211.	Nabumetone	7.80	25	229.1	171.1	128.1
212.	Oxaprozin	7.87	25	294.1	103.0	234.2
213.	HU-211	8.03	25	387.3	155.9	128.1
214.	JWH‐200	8.04	25	385.3	155.0	127.1
215.	Diazepam	8.04	25	285.1	192.9	153.9
216.	Mefenamic acid	8.04	25	242.1	224.1	-
217.	Tolfenamic acid*	8.17	50	259.9	215.8	-
218.	Meclofenamic acid*	8.18	200	293.9	257.8	177.7
219.	(+/-)-CP 47,497*	8.27	200	317.1	245.1	159.0
220.	Indomethacin*	8.29	50	356.0	311.8	296.9
221.	Perampanel	8.32	25	350.2	219.1	247.1
222.	(+/-)-CP 47,497 C8 homologue*	8.42	200	331.1	259.0	159.1
223.	11-Nor-9-carboxy-delta-9-THC*	8.44	25	343.0	298.9	244.8
224.	Prazepam	8.51	25	325.2	271.0	139.9
225.	XLR-11	8.60	25	330.3	125.2	144.0
226.	Δ9-THC	8.76	25	315.2	193.0	123.2
227.	UR-144	8.78	25	312.3	125.1	214.1
228.	JWH-250	8.96	25	336.3	120.9	91.1
229.	THCA-A*	9.12	25	357.1	312.9	245.0
230.	JWH-073	9.21	25	328.2	155.0	127.1
231.	JWH‐018	9.35	25	342.3	127.1	155.1

*Analyzed using negative polarity.

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

30 °C

Standard/Sample

Diluent:

Water

Inj. Vol.:

5 µL

Mobile Phase

Water + 0.1% formic acid + 2 mM ammonium formate

Methanol + 0.1% formic acid + 2 mM ammonium formate

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	95	5
9.00	0.6	0	100
10.00	0.6	0	100
10.01	0.6	95	5
12.00	0.6	95	5

Detector	MS/MS
Ion Mode:	ESI+/ESI-
Mode:	Scheduled MRM
Instrument	UHPLC
Notes	1. Positive and negative polarity data were collected simultaneously from a single injection. 2. Ibuprofen, fosphenytoin, and topiramate degrade quickly in solution. 3. Levorphanol and dextrorphan are enantiomers and are not resolved. 4. Escitalopram is a pure enantiomer and citalopram is a racemic mixture of both enantiomers. These compounds are not resolved. 5. DMAA consists of 4 stereoisomers, 2 of which are partially resolved. 6. Doxepin and desmethylydoxepin consist of a mixture of cis/trans isomers. The isomers are fully resolved and the retention time identifies the main isomer component. 7. Lornoxicam, meloxicam, etodolac, oxaprozin, indomethacin, and mefenamic acid can be detected by positive or negative polarity. The polarity chosen in this chromatogram provides optimal response.

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