Metanephrines on Raptor FluoroPhenyl by LC-MS/MS

LC_CF0642

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Normetanephrine	1.15	166.1	121.1	134.0
2.	Metanephrine	1.52	180.1	165.1	148.3

Column

Raptor FluoroPhenyl (cat.# 9319A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

30 °C

Standard/Sample

Conc.:

20 ng/mL in water

Inj. Vol.:

5 µL

Mobile Phase

A:

Water + 0.2% formic acid

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	98	2
2.00	0.4	60	40
2.01	0.4	98	2
6.00	0.4	98	2

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC

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