25-Hydroxyvitamin D2/D3 and 3-Epi-25-Hydroxyvitamin D2/D3 on Raptor FluoroPhenyl by LC-MS/MS

LC_CF0644

Column

Raptor FluoroPhenyl (cat.# 9319A1E)

Dimensions:

100 mm x 3 mm ID

Particle Size:

2.7 µm

Temp.:

30 °C

Standard/Sample

Diluent:

Water:methanol (50:50)

Conc.:

25-50 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	25	75
4.00	0.6	15	85
4.10	0.6	0	100
5.00	0.6	0	100
5.01	0.6	25	75
7.00	0.6	25	75

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	HPLC

Download PDF