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Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|
1. | Prednisolone | 2.53 | 361.2 | 343.2 | 325.1 |
2. | Prednisone | 3.07 | 359.1 | 341.1 | 147.1 |
3. | Betamethasone | 4.64 | 393.1 | 373.1 | 91.2 |
4. | Dexamethasone | 4.87 | 393.1 | 373.1 | 355.2 |
Column | Raptor Biphenyl (cat.# 9309A12) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||
Particle Size: | 2.7 µm | ||||||||||||
Pore Size: | 90 Å | ||||||||||||
Temp.: | 40 °C | ||||||||||||
Standard/Sample | |||||||||||||
Diluent: | Water:methanol (80:20) | ||||||||||||
Conc.: | 20 ng/mL | ||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||
Mobile Phase | |||||||||||||
A: | Water + 0.1% formic acid | ||||||||||||
B: | Acetonitrile + 0.1% formic acid | ||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
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