Glucocorticoid Isomers on Raptor Biphenyl by LC-MS/MS

LC_CF0659

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Prednisolone	2.53	361.2	343.2	325.1
2.	Prednisone	3.07	359.1	341.1	147.1
3.	Betamethasone	4.64	393.1	373.1	91.2
4.	Dexamethasone	4.87	393.1	373.1	355.2

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

Water:methanol (80:20)

Conc.:

20 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

Water + 0.1% formic acid

B:

Acetonitrile + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	75	25
6.00	0.6	75	25

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC

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