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Xanthines on Force FluoroPhenyl by LC/MS/MS

LC_CF0672
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Theobromine1.5350181.13137.95163.01
2.Theophylline1.7950181.13124.0296.03
3.Paraxanthine1.9550181.13124.0296.03
4.Caffeine2.3350195.09138.01110.03
ColumnForce FluoroPhenyl (cat.# 9639212)
Dimensions:100 mm x 2.1 mm ID
Particle Size:1.8 µm
Temp.:40 °C
Sample
Diluent:70:30 Water:methanol
Conc.:50 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.37030
2.50.33070
3.00.30100
3.010.37030
5.00.37030
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC