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Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|---|
1. | Theobromine | 1.53 | 50 | 181.13 | 137.95 | 163.01 |
2. | Theophylline | 1.79 | 50 | 181.13 | 124.02 | 96.03 |
3. | Paraxanthine | 1.95 | 50 | 181.13 | 124.02 | 96.03 |
4. | Caffeine | 2.33 | 50 | 195.09 | 138.01 | 110.03 |
Column | Force FluoroPhenyl (cat.# 9639212) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 1.8 µm | ||||||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | 70:30 Water:methanol | ||||||||||||||||||||||||
Conc.: | 50 ng/mL | ||||||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||||||
B: | Methanol | ||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
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