Xanthines on Force FluoroPhenyl by LC/MS/MS

LC_CF0672

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Theobromine	1.53	50	181.13	137.95	163.01
2.	Theophylline	1.79	50	181.13	124.02	96.03
3.	Paraxanthine	1.95	50	181.13	124.02	96.03
4.	Caffeine	2.33	50	195.09	138.01	110.03

Column

Force FluoroPhenyl (cat.# 9639212)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

1.8 µm

Temp.:

40 °C

Standard/Sample

Diluent:

70:30 Water:methanol

Conc.:

50 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.3	70	30
2.5	0.3	30	70
3.0	0.3	0	100
3.01	0.3	70	30
5.0	0.3	70	30

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC

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