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Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | Polarity | |
---|---|---|---|---|---|---|
1. | Digoxin | 0.95 | 798.4 | 651.4 | 355.1 | + |
2. | Flecainide | 1.28 | 415.3 | 397.9 | 300.9 | + |
3. | Furosemide | 1.32 | 329.0 | 205.2 | 285.1 | - |
4. | Verapamil | 1.68 | 455.1 | 165.2 | 150.0 | + |
5. | Digitoxin | 1.85 | 782.4 | 635.2 | 243.1 | + |
6. | Amiodarone | 3.17 | 646.1 | 58.1 | 86.2 | + |
Column | Force Biphenyl (cat.# 9629252) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 1.8 µm | ||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | Water:acetonitrile (90:10) | ||||||||||||||||||||
Conc.: | 100 ng/mL | ||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | Water + 5 mM ammonium formate + 0.1% formic acid | ||||||||||||||||||||
B: | Acetonitrile + 0.1% formic acid | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+/ESI- |
Mode: | Scheduled MRM |
Instrument | UHPLC |
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