Cardiac Drugs on Force Biphenyl by LC-MS/MS

LC_CF0675

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion	Polarity
1.	Digoxin	0.95	798.4	651.4	355.1	+
2.	Flecainide	1.28	415.3	397.9	300.9	+
3.	Furosemide	1.32	329.0	205.2	285.1	-
4.	Verapamil	1.68	455.1	165.2	150.0	+
5.	Digitoxin	1.85	782.4	635.2	243.1	+
6.	Amiodarone	3.17	646.1	58.1	86.2	+

Column

Force Biphenyl (cat.# 9629252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Temp.:

40 °C

Standard/Sample

Diluent:

Water:acetonitrile (90:10)

Conc.:

100 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

A:

Water + 5 mM ammonium formate + 0.1% formic acid

B:

Acetonitrile + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.3	60	40
3.50	0.3	5	95
3.51	0.3	60	40
5.5	0.3	60	40

Detector	MS/MS
Ion Mode:	ESI+/ESI-
Mode:	Scheduled MRM
Instrument	UHPLC

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