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Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|
1. | Morphine-3-β-D-glucuronide | 0.70 | 462.1 | 286.0 | 152.1 |
2. | Morphine | 1.26 | 286.1 | 164.9 | 152.0 |
3. | Morphine-6-β-D-glucuronide | 1.34 | 462.1 | 286.0 | 152.1 |
4. | Morphine-N-oxide | 1.41 | 302.1 | 284.9 | 161.9 |
5. | 6-Acetylmorphine | 1.86 | 328.1 | 211.1 | 165.0 |
Column | Force Biphenyl (cat.# 9629252) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 1.8 µm | ||||||||||||||||||||||||
Temp.: | 35 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | 0.1% Formic acid in water | ||||||||||||||||||||||||
Conc.: | 50 ng/mL | ||||||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||||||
B: | 0.1% Formic acid in methanol | ||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
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