Morphine and Metabolites on Force Biphenyl by UHPLC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Morphine-3-β-D-glucuronide	0.70	462.1	286.0	152.1
2.	Morphine	1.26	286.1	164.9	152.0
3.	Morphine-6-β-D-glucuronide	1.34	462.1	286.0	152.1
4.	Morphine-N-oxide	1.41	302.1	284.9	161.9
5.	6-Acetylmorphine	1.86	328.1	211.1	165.0

Column

Force Biphenyl (cat.# 9629252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Temp.:

35 °C

Sample

Diluent:

0.1% Formic acid in water

Conc.:

50 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	85	15
0.50	0.5	85	15
2.00	0.5	30	70
2.01	0.5	85	15
4.00	0.5	85	15

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