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|Precursor Ion||Product Ion||Product Ion|
|Column||Raptor FluoroPhenyl (cat.# 9319A12)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9319A0252)|
|Inj. Vol.:||10 µL|
|A:||0.1% Formic acid in water|
|Max Pressure:||400 bar|
|Sample Preparation||The sample solution was prepared by protein precipitation using Resprep PPT3 96-well plates (cat# 26489) and reservoir (cat# 26493). A 200 μL aliquot of fortified human plasma was mixed with 600 μL of acetonitrile by vortexing for 30 seconds at 2000 rpm. The sample was filtered using a vacuum manifold. The filtrate was dried and reconstituted with 100 μL of water:methanol (95:5) and injected for analysis.|
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