Analysis of Antiretroviral Drugs on Raptor Biphenyl by LC-MS/MS

LC_CF0679

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Ganciclovir	0.57	256.0	152.1	134.9
2.	Lamivudine	0.85	230.0	112.0	95.1
3.	Zidovudine	1.54	268.1	127.2	110.1
4.	Nevirapine	2.16	267.3	225.9	196.9
5.	Efavirenz	2.67	316.1	243.8	168.1
6.	Ritonavir	2.99	721.5	140.1	296.0

Column

Raptor Biphenyl (cat.# 9309A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

30 °C

Standard/Sample

Custom mix

Diluent:

Water + 0.01% acetic acid

Conc.:

100 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

A:

Water + 0.01% acetic acid

B:

Methanol + 0.01% acetic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	98	2
3.00	0.8	0	100
3.20	0.8	0	100
3.21	0.8	98	2
5.00	0.8	98	2

Max Pressure:

500 bar

Detector	MS/MS
Ion Mode:	ESI+
Mode:	Scheduled MRM
Instrument	UHPLC

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