Methylmalonic Acid (MMA) on Force C18 by LC-MS

LC_CF0696

	Peaks	t_R (min)	SIM
1.	Succinic acid	0.553	117.0
2.	Methylmalonic acid (MMA)	0.719	117.0

Column

Force C18 (cat.# 9634252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Diluent:

Water

Conc.:

20 µg/mL

Inj. Vol.:

2 µL

Mobile Phase

A:

Water, 0.5% formic acid

B:

Methanol, 0.5% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	95	5
1.50	0.5	95	5
1.51	0.5	5	95
2.00	0.5	5	95
2.01	0.5	95	5
4.00	0.5	95	5

Max Pressure:

750 bar

Detector	MS
Acquisition Type:	SIM
Interface:	ESI-
Instrument	UHPLC

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