Steroid Panel Analysis on Force Biphenyl

LC_CF0702

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	Cortisol	2.3	363.3	121.2	327.2
2.	Estradiol	2.6	255.3	159.1	133.1
3.	11-Deoxycortisol	4.0	347.4	109.2	97.1
4.	DHEA	4.3	271.3	253.2	213.2
5.	Boldenone	4.4	287.2	121.2	135.2
6.	Corticosterone	4.4	347.4	329.2	121.1
7.	Stanozolol	4.4	329.3	81.1	95.1
8.	Epitestosterone	4.5	289.2	109.2	97.1

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
9.	17-OH-progesterone	4.8	331.3	97.2	109.5
10.	Testosterone	5.0	289.2	109.1	97.0
11.	Trenbolone	5.1	303.3	109.2	97.1
12.	Methyltestosterone	5.1	271.2	253.1	199.2
13.	Nandrolone	5.3	275.2	109.2	257.3
14.	25-Hydroxyvitamin D3	6.1	383.3	365.3	107.1
15.	Androstenedione	6.2	287.2	97.1	109.2
16.	Progesterone	7.5	315.3	109.2	97.1

Column

Force Biphenyl LC Column (cat.# 9629512)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Steroids panel

Diluent:

50:50 Methanol:water

Conc.:

5 ng/mL

Inj. Vol.:

50 µL

Mobile Phase

A:

Water, 0.1% formic acid

B:

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	30	70
8.00	0.4	0	100
8.01	0.4	30	70
10.00	0.4	30	70

Max Pressure:

250 bar

Detector	MS/MS
Ion Mode:	ESI+
Mode:	Scheduled MRM
Instrument	UHPLC
Notes

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