Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine on Raptor EtG/EtS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Ethyl-β-D-glucuronide-d5	0.86	225.9	84.9	-
2.	Ethyl-β-D-glucuronide	0.87	220.8	84.9	74.8
3.	Ethyl sulfate-d5	2.14	129.7	97.7	-
4.	Ethyl sulfate	2.17	124.7	96.8	79.7

Column

Raptor EtG/EtS (cat.# 9325A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)

Temp.:

35 °C

Standard/Sample

Diluent:

0.1% Formic acid in water

Conc.:

A 500 ng/mL standard was prepared in urine. 50 µL of the standard was diluted with 950 µL of a working internal standard (25 ng/mL EtS-d5/100 ng/mL EtG-d5 in 0.1% formic acid in water). The sample was vortexed at 3,500 rpm for 10 seconds to mix. The sample was then centrifuged at 3,000 rpm for 5 minutes at 10 °C. The autosampler needle was adjusted to inject from the supernatant.

Inj. Vol.:

10 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	95	5
2.50	0.5	65	35
2.51	0.5	95	5
4.00	0.5	95	5

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	HPLC
Notes	Reference Standards Ethyl-β-D-glucuronide (cat.# 34101) Ethyl-β-D-glucuronide-d5 (cat.# 34102) Ethyl sulfate sodium salt (cat.# 34103) Ethyl sulfate-d5 sodium salt (cat.# 34104)

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