Fentanyl and Analogues in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Norfentanyl-d5	1.03	1	238.30	84.15	-
2.	Norfentanyl	1.04	10	233.27	84.15	56.06
3.	Remifentanil	1.34	10	377.37	113.15	317.30
4.	Acetyl fentanyl-13C6	1.69	1	329.37	188.25	-
5.	Acetyl fentanyl	1.69	10	323.37	188.25	105.15
6.	Alfentanil	1.88	10	417.47	268.31	197.23
7.	Fentanyl-d5	1.95	1	342.47	188.27	-
8.	Fentanyl	1.96	10	337.37	188.26	105.08
9.	Carfentanil-d5	2.04	1	400.40	340.41	-
10.	Carfentanil	2.05	10	395.40	113.14	335.35
11.	Butyryl fentanyl	2.15	10	351.43	188.20	105.15
12.	Sufentanil-d5	2.25	1	392.40	238.25	-
13.	Sufentanil	2.26	10	387.40	238.19	111.06

Column

Raptor Biphenyl (cat.# 9309552)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)

Temp.:

40 °C

Standard/Sample

Diluent:

70:30 Water:methanol

Inj. Vol.:

5 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	70	30
2.50	0.4	30	70
2.51	0.4	70	30
3.50	0.4	70	30

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	Human urine was fortified at 10 ng/mL with target analytes. An 80 μL urine aliquot was mixed with 320 μL of 70:30 water:methanol solution (5-fold dilution) and 10 μL of internal standard solution (40 ng/mL in methanol) in a Thomson SINGLE StEP filter vial (Restek cat.# 25895). After filtering through the 0.2 µm PVDF membrane, 5 μL was injected for analysis.

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