Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.

Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Creatine and Creatinine in Human Plasma on Raptor HILIC-Si by LC-MS/MS

PeakstR (min)Precursor IonProduct IonProduct Ion
ColumnRaptor HILIC-Si (cat.# 9310A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter 0.2 µm (cat.# 25810)
Temp.:40 °C
Diluent:20:80 Water:acetonitrile
Conc.:Endogenous levels
Inj. Vol.:0.2 µL
Mobile Phase
A:5 mM Ammonium formate in 20:80 water:acetonitrile
Time (min)Flow (mL/min)%A
Ion Mode:ESI+
NotesSample Preparation:
Endogenous levels of creatinine and creatine in human plasma were determined using a single protein precipitation step followed by LC-MS/MS analysis. A 50 μL aliquot of human plasma (K2EDTA) was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4,300 rpm for 10 min, 200 μL of the supernatant was transferred to a new vial and mixed with 50 μL of water. Centrifugation was performed again before injection.