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|Column||Raptor HILIC-Si (cat.# 9310A52)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter, 0.2 µm (cat.# 25810)|
|Inj. Vol.:||0.2 µL|
|A:||5 mM Ammonium formate in 20:80 water:acetonitrile|
|Sample Preparation||Endogenous levels of creatinine and creatine in human plasma were determined using a single protein precipitation step followed by LC-MS/MS analysis. A 50 μL aliquot of human plasma (K2EDTA) was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4300 rpm for 10 min, 200 μL of the supernatant was transferred to a new vial and mixed with 50 μL of water. Centrifugation was performed again before injection. |