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|Peaks||tR (min)||Precursor Ion||Product Ion||Product Ion|
|Column||Raptor HILIC-Si (cat.# 9310A52)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter, 0.2 µm (cat.# 25810)|
|Inj. Vol.:||0.2 µL|
|A:||5 mM Ammonium formate in 20:80 water:acetonitrile|
|Sample Preparation||Endogenous levels of creatinine and creatine in human urine were determined using a simple dilute-and-shoot method. A 50 μL aliquot of human urine was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4300 rpm for 10 min, 10 μL of the supernatant was added to the 1490 μL of 80% acetonitrile in water. Centrifugation was performed again before injection.|
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