Creatine and Creatinine in Human Urine on Raptor HILIC-Si by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Creatinine	0.537	114.0	44.3	86.0
2.	Creatine	1.240	132.1	43.3	90.2

Column

Raptor HILIC-Si (cat.# 9310A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

UltraShield UHPLC precolumn filter, 0.2 µm (cat.# 25810)

Temp.:

40 °C

Standard/Sample

Diluent:

20:80 Water:acetonitrile

Conc.:

Endogenous levels

Inj. Vol.:

0.2 µL

Mobile Phase

5 mM Ammonium formate in 20:80 water:acetonitrile

Time (min)	Flow (mL/min)	%A
0.00	0.5	100
1.5	Stop

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	Endogenous levels of creatinine and creatine in human urine were determined using a simple dilute-and-shoot method. A 50 μL aliquot of human urine was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4300 rpm for 10 min, 10 μL of the supernatant was added to the 1490 μL of 80% acetonitrile in water. Centrifugation was performed again before injection.

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