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|Column||Raptor EtG/EtS (cat.# 9325A12)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter (cat.# 25809)|
|Ethyl sulfate sodium salt (EtS) (cat.# 34103)|
|Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104)|
|Diluent:||0.1% Formic acid in water|
|Inj. Vol.:||10 µL|
|A:||0.1% Formic acid in water|
|B:||0.1% Formic acid in acetonitrile|
|Sample Preparation||A 500 ng/mL standard was prepared in oral fluid. 50 µL of the standard was diluted with 950 µL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C. The autosampler needle was adjusted to inject from the supernatant.|