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|Precursor Ion||Product Ion||Product Ion|
|Column||Raptor EtG/EtS (cat.# 9325A12)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)|
|Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104)|
|Diluent:||0.1% Formic acid in water|
|Conc.:||UTAK EtG Plus urine quality control (200 ng/mL EtS and 500 ng/mL EtG)|
|Inj. Vol.:||10 µL|
|A:||0.1% Formic acid in water|
|B:||0.1% Formic acid in acetonitrile|
|Sample Preparation||UTAK EtG Plus Level 1 control contains EtG at 500 ng/mL and EtS at 200 ng/mL concentrations. A 50 μL aliqout was taken from the the Level 1 control and diluted 20 times with 950 μL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C and 10 μL was injected for LC-MS/MS analysis.|
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