EtG/EtS in UTAK EtG Plus Level 1 Urine Control on Raptor EtG/EtS by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Ethyl-β-D-glucuronide	0.81	500	221.20	75.1	85.1
2.	Ethyl-β-D-glucuronide-d5	0.81	100	226.20	85.00	-
3.	Ethyl sulfate-d5	1.84	100	130.10	98.05	-
4.	Ethyl sulfate	1.87	200	125.10	97.10	80.00

Column

Raptor EtG/EtS (cat.# 9325A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)

Temp.:

35 °C

Standard/Sample

Ethyl-β-D-glucuronide-d5 (EtG-d5) (cat.# 34102)

Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104)

Diluent:

0.1% Formic acid in water

Conc.:

UTAK EtG Plus urine quality control (200 ng/mL EtS and 500 ng/mL EtG)

Inj. Vol.:

10 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	95	5
2.50	0.5	65	35
2.51	0.5	95	5
4.00	0.5	95	5

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	HPLC
Sample Preparation	UTAK EtG Plus Level 1 control contains EtG at 500 ng/mL and EtS at 200 ng/mL concentrations. A 50 μL aliqout was taken from the the Level 1 control and diluted 20 times with 950 μL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C and 10 μL was injected for LC-MS/MS analysis.

Download PDF