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|Column||Force C18 (cat.# 963431E)|
|Dimensions:||100 mm x 3.0 mm ID|
|Particle Size:||3 µm|
|Pore Size:||100 Å|
|Guard Column:||Force C18 EXP guard column cartridge 5 mm, 3.0 mm ID, 3 µm (cat.# 963450253)|
|Inj. Vol.:||10 µL|
|A:||0.5% Formic acid in water|
|B:||0.5% Formic acid in methanol|
|Sample Preparation||100 μL of whole human blood was fortified at 50 μg/mL with GHB, GBL, 1,4-BD, and GHB-D6 (IS) using 5 μL of 1 mg/mL solutions. The blood was precipitated with 380 μL methanol. The sample was then vortexed at 1000 rpm for 10 seconds and centrifuged at 3000 rpm for 10 minutes at 10 °C. 50 μL of the supernatant was removed and diluted to 1 mL with water. The sample was then vortexed and subjected to LC-MS/MS analysis. (Internal standard not shown on chromatogram.)|