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|Peaks||tR (min)||Conc.||Precursor Ion||Product Ion|
|1.||Phosphatidylethanol-d5 (PEth-d5) 16:0/18:1||1.58||0.4 μM||706.6||281.3|
|2.||Phosphatidylethanol (PEth) 16:0/18:1||1.58||0.5 μM||701.5||255.3|
|Column||Raptor FluoroPhenyl (cat.# 9319A52)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Conc.:||0.5 μM in blood|
|Inj. Vol.:||2 µL|
|A:||Water, 5 mM ammonium acetate|
|Notes||Sample Preparation Procedure (Provided by Redhot Diagnostics):|
The fortified blood sample (50 µL) was mixed with 50 µL of internal standard (0.4 µM PEth-d5 in 2-propanol) and 150 µL of 4:1 2-propanol:tetrahydrofuran. The mixture was vortexed for 20 seconds at 3000 rpm and centrifuged for 10 minutes at 4300 rpm. The supernatant (2 µL) was injected for analysis.